The Microarray lab is located at floor 3 (in the C part). There you can perform quality control of you RNA, labelling of RNA, hybridisations of arrays etc. We also have two Scan Array scanners (Perkin Elmer)which together with the Genepix or Genespring software provide a powerful, but easy to use, platform for initial micro array analysis.
The Genomics lab provide two types of arrays:
POP2 – a 25k array of Populus cDNAs
CATMA – a 25k array of Arabidopsis GSTs
Users of POP2 arrays (the PIs of the group) need to fill in a request form before they are allowed to use any slides. This is done electronically on the MicroPop, our local BASE site. Here you also fill in all data about your experiments.
The CATMA project is funded by Wallenberg Consortium North.
There are various protocols available for the analysis of microarrays. Some currently used at UPSC are found here.
- Coupling of microarray samples ASP-version
- cDNA microarray of small plant tissue samples using a cDNA tag target amplification
- Labelling of cDNA target prepared according to Hertzberg Amp Modified protocol
- Hybridization of microarray samples ASP-version
- Manual hybridization on slides using lifterslips
- Protocol for total RNA extraction from stem
- Extraction of RNA from Populus leaves
- RNA extraction (small samples)
The Genomics lab has a BioRobotics MicroGrid II robot, capable of printing over 100 slides/run using samples stored in a temperature controlled biobank. Using a 24 pin tool, the robot is able to produce high density slide arrays.
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